ABSTRACT
ABSTRACT Objective: To analyze the evidence available in the literature on the use of essential oils for healing and/or preventing infection in surgical wounds. Method: Systematic review according to the JBI model and PRISMA statement. The search was carried out in November/2020 and updated in December/2021, using descriptors and keywords, in the CINAHL, LILACS, CENTRAL, EMBASE, PUBMED, Scopus, and Web of Science databases. The quality of the evidence was assessed using the JBI critical appraisal tool for randomized controlled trials. Results: Five publications were included. Three studies evaluated healing and the presence of infection after episiotomy using the Redness-Edema-Ecchymosis-Discharge-Approximation (REEDA) scale; one study evaluated healing after periodontal surgery using the plaque index and Modified Gingival Index; the other four studies considered the presence of infection after episiotomy. Most studies used lavender oil, associated or not with other oils (80%). Two studies showed an improvement in healing. The infection outcome, although mentioned by 60% of studies, was not assessed as a primary outcome. Conclusion: The promising efficacy of essential oils, especially lavender, was verified in the healing of surgical wounds, especially in episiotomies.
RESUMEN Objetivo: Analizar la evidencia en la literatura sobre el uso de aceites esenciales para la cicatrización y/o prevención de infecciones en heridas quirúrgicas. Método: Revisión sistemática de acuerdo con el modelo JBI y el PRISMA. Se realizó una búsqueda en las bases de datos CINAHL, LILACS, CENTRAL, EMBASE, PUBMED, Scopus y Web of Science, en el periodo de noviembre/2020, con actualizaciones en diciembre/2021, utilizando descriptores y palabras clave. La calidad de la evidencia se evaluó por la herramienta JBI critical appraisal para ensayos controlados aleatorizados. Resultados: Se incluyeron cinco publicaciones. Tres estudios evaluaron la curación y la presencia de infección tras episiotomía mediante la escala REEDA (Redness-Edema-Ecchimosis-Discharge-Approximation); uno evaluó la curación después de cirugía periodontal utilizando el índice de placa y el índice gingival modificado; y los demás consideraron la presencia de infección posterior a la episiotomía. La mayoría de los estudios utilizaron aceite de lavanda, asociado a otros aceites o no (80%). Dos estudios demostraron mejorar la cicatrización. El resultado infección, aunque mencionado por el 60% de los estudios, no se evaluó como resultado primario. Conclusión: Se verificó la prometedora eficacia de los aceites esenciales, especialmente el de lavanda, en la cicatrización de heridas quirúrgicas, especialmente en episiotomías.
RESUMO Objetivo: Analisar as evidências disponíveis na literatura sobre o uso de óleos essenciais para a cicatrização e/ou prevenção de infecção em feridas cirúrgicas. Método: Revisão sistemática segundo modelo JBI e declaração PRISMA. Busca realizada em novembro/2020 e atualizada em dezembro/2021, utilizando-se descritores e palavras-chave, nas bases CINAHL, LILACS, CENTRAL, EMBASE, PUBMED, Scopus e Web of Science. A qualidade das evidências foi avaliada usando a ferramenta JBI critical appraisal para ensaios clínicos randomizados. Resultados: Cinco publicações foram incluídas. Três estudos avaliaram a cicatrização e presença de infecção após episiotomia por meio da escala REEDA (Redness-Edema-Ecchymosis-Discharge-Approximation); um avaliou cicatrização após cirurgia periodontal por meio do índice de placa e Índice Gengival Modificado; o restante considerou a presença de infecção após episiotomia. A maioria dos estudos utilizou o óleo de lavanda, associado ou não a outros óleos (80%). Em dois estudos houve melhora da cicatrização. O desfecho infecção, embora mencionado por 60% estudos, não foi avaliado como primário. Conclusão: Verificou-se a eficácia promissora de óleos essenciais, sobretudo do de lavanda, na cicatrização de feridas cirúrgicas, especialmente em episiotomias.
Subject(s)
Oils, Volatile , Surgical Wound , Wound Healing , Wound Infection , Aromatherapy , LavandulaABSTRACT
The objective was to evaluate the production and composition of lavender essential oil, simultaneously at open field and greenhouse, in spring-summer season, under different types of fertilization. Each cultivation system was considered an experiment (field and greenhouse), and in both, the experimental design used was randomized blocks, with five treatments, being fertilization with nutrient source -100% mineral (100%M), 100% organic (100%O), 100% organomineral (100%OM), 50% organomineral (50%OM), and without fertilization (control), and three repetitions. Better performance and productivity of Lavandula dentatais related to respectively 100% OM and 50% OM fertilization in the field and greenhouse. The essential oil content does not differ between cultivation systems and kind of fertilizers, and the majorities compounds were 1.8-cineol, fenchone and camphor.
El objetivo fue evaluar la producción y composición del aceite esencial de lavanda a campo abierto e invernadero simultáneamente, en la temporada primavera-verano, bajo diferentes tipos de fertilización. Cada sistema de cultivo fue considerado un experimento (campo e invernadero), y en ambos, el diseño experimental utilizado fue bloques al azar, con cinco tratamientos, siendo la fertilización con fuente de nutrientes 100% mineral (100% M), 100% orgánico (100% O), 100% organomineral (100% MO), 50% organomineral (50% MO), y sin fertilización (control), y tres repeticiones. Un mejor rendimiento y productividad de Lavandula dentata está relacionado con una fertilización de 100%OM y 50% OM en el campo y en invernadero, respectivamente. El contenido de aceite esencial no difiere entre sistemas de cultivo y tipo de fertilizante, y los compuestos mayoritarios fueron 1.8-cineol, fenchone y alcanfor.
Subject(s)
Plant Oils/metabolism , Plant Oils/chemistry , Oils, Volatile/metabolism , Oils, Volatile/chemistry , Lavandula/chemistry , Crop Production , Nutrients , Monoterpenes/analysis , Greenhouses , FertilizationABSTRACT
ABSTRACT Objective To analyze the effect of lavender essential oil associated with massage on spinal pain levels in obese women. Methods The sample included 49 obese women, between 20 and 60 years of age, randomly assigned to three groups: control (n=15), intervention (n=19) and placebo (n=15). The intervention and placebo groups were submitted to eight 35-minute sessions, twice a week for one month, but the essential oil of Lavandula angustifolia was used only in the intervention group. A placebo was used for the placebo group and the control group received no intervention. Pain levels were measured before and after the intervention using the Visual Analog Scale. Results There was a significant decrease in total spinal pain (p=0.004), in the cervical region (p=0.003) and in the lumbar region (p=0.008) in the intervention group. Conclusion Lavender essential oil had a positive impact on the reduction of pain in the spine of obese women, as well as in the specific areas of the cervical and lumbar regions compared to the control and placebo groups. Level of evidence I; Randomized clinical trial.
RESUMO Objetivo Analisar o efeito do óleo essencial de lavanda associado à massagem nos níveis de dor na coluna em mulheres obesas. Métodos A amostra incluiu 49 mulheres obesas com idades entre 20 e 60 anos, randomizadas em três grupos: controle (n = 15), intervenção (n = 19) e placebo (n = 15). Os grupos intervenção e placebo foram submetidos a oito sessões com duração de 35 minutos, duas vezes por semana durante um mês, sendo que o óleo essencial de Lavandula angustifolia foi usado apenas no grupo intervenção. Um placebo foi usado para o grupo placebo e o grupo controle não recebeu intervenção. Os níveis de dor foram medidos antes e depois da intervenção pela Escala Visual Analógica. Resultados Houve diminuição significativa da dor total na coluna (p = 0,004), na região cervical (p = 0,003) e na região lombar (p = 0,008) no grupo intervenção. Conclusão O óleo essencial de lavanda teve impacto positivo na redução da dor na coluna de mulheres obesas, bem como em áreas específicas das regiões cervical e lombar em comparação com os grupos controle e placebo. Nível de evidência I; Ensaio clínico randomizado.
RESUMEN Objetivo Analizar el efecto del aceite esencial de lavanda asociado al masaje sobre los niveles de dolor espinal en mujeres obesas. Métodos La muestra incluyó a 49 mujeres obesas con edades de 20 a 60 años, distribuidas aleatoriamente en tres grupos: control (n = 15), intervención (n = 19) y placebo (n = 15). Los grupos intervención y placebo se sometieron a ocho sesiones con 35 minutos de duración, dos veces por semana durante un mes, utilizándose el aceite esencial de Lavandula angustifolia solo en el grupo intervención. Para el grupo placebo se utilizó un placebo y el grupo control no recibió ninguna intervención. Los niveles de dolor se midieron antes y después de la intervención, utilizando la Escala Visual Analógica. Resultados Hubo una disminución significativa del dolor total en la columna (p = 0,004), en la región cervical (p = 0,003) y en la región lumbar (p = 0,008) en el grupo intervención. Conclusión El aceite esencial de lavanda tuvo un impacto positivo en la reducción del dolor de columna vertebral en mujeres obesas, así como en las áreas específicas de las regiones cervical y lumbar en comparación con los grupos control y placebo. Nivel de evidencia I; Ensayo clínico aleatorizado.
Subject(s)
Humans , Congenital Abnormalities , Minimally Invasive Surgical Procedures , Neuromuscular DiseasesABSTRACT
Abstract Background Aromatherapy consists in the use of volatile aromatic compounds of plant essential oils. Application methods include massage, baths, and mainly inhalation. Lavender essential oil is considered the most effective treatment for emotional disorders, such as stress and anxiety, due to its anxiolytic and sedative agents, which are known to interfere with physiological cardiovascular reactions. Objectives To investigate the effects of aromatherapy using lavender essential oil on hemodynamic responses and emotional aspects of patients with cardiovascular diseases. Methods A systematic review was conducted using Embase, Bireme, MEDLINE, PEDro, and Scopus electronic databases. Randomized clinical trials that evaluated hemodynamic and emotional outcomes using interventions with lavender essential oil in patients with cardiovascular diseases were selected. Of 539 studies initially identified, 51 were read in full and only 5 were eligible for inclusion. Results Reductions were demonstrated in hemodynamic responses, such as systolic and diastolic blood pressure, mean arterial pressure, and heart rate, as well as a decrease in anxiety, depression, stress, and fatigue compared with the control group. Statistical significance was set at p<0.05. Conclusion: Aromatherapy with the use of lavender essential oil provided benefits to hemodynamic parameters, such as anxiety, stress, depression, and fatigue levels, in patients with cardiovascular diseases. (Int J Cardiovasc Sci. 2020; [online].ahead print, PP.0-0)
Subject(s)
Humans , Male , Female , Cardiovascular Diseases/therapy , Aromatherapy , Lavandula , Anxiety , Oils, Volatile , Cardiovascular Diseases/psychology , Depression , Fatigue , Hemodynamics/drug effectsABSTRACT
OBJECTIVE：To investigate the composition-activity relationship of the antioxidant active component in essential oil of Lavandula angustifolia from Xinjiang. METHODS ：Antioxidant activity of essential oil of L. angustifolia was evaluated with 1，1-diphenyl-2-picrylhydrazyl（DPPH）free radicals scavenging method. GC-MS combined with retention index were adopted to qualitatively analyze the compounds. The relationship of chemical components with antioxidant activity of essential oil of L. angustifolia was analyzed by principal component analysis （PCA） and partial least squares analyses （PLS）. The variable importance in projection （VIP＞1）was used to screen the components which had great contribution to the elimination of DPPH free radical. RESULTS ：The IC 50 values of essential oil in 9 batches of L. angustifolia from 3 different varieties was 4.82-9.88 mg/mL，IC50 of France blue was 6.66-8.58 mg/mL，IC50 of H- 701 and Xinxun- 4 were 4.82-7.73 mg/mL and 9.55-9.88 mg/mL，respectively. A total of 40 chemical components were identified of essentil oil in 9 batches of L. angustifolia . Results of PCA analysis showed that accumulative variance contribution rate of former 2 main components was 81.8％；9 batches of samples were divided into 3 regions， and different varieties were classified into one category ；the characteristic variable components of the essential oil of French blue samples were lavandulyl acetate corresponding to peak 32 and trans- β-ocimene corresponding to peak 9；the characteristic variable components of the essential oil of H- 701 samples were linalool corresponding to peak 16，linalyl acetate corresponding to peak 30 and terpinen- 4-ol corresponding to peak 21；the characteristic variable components of the essential oil of Xinxun- 4 samples were eucalyptol corresponding to peak 8，camphor corresponding to peak 18 and 2-borneol corresponding to peak 20. The compound with the greatest antioxidant activity was linalool corresponding to peak 16 （VIP＝2.940 0），followed by terpenein- 4-ol corresponding to peak 21（VIP＝2.863 1），caryophyllene corresponding to peak 35（VIP＝2.570 8）and eucalyptol corresponding to peak 8（VIP＝2.115 8）. CONCLUSIONS ：The essential oil of L. angustifolia has certain antioxidant activity ，and H- 701 sample has higher antioxidant activity. Linalool corresponding to peak 16 is the most important component of essential oil of L. angustifolia in eliminating DPPH free radical.
ABSTRACT
Lavender is well-known for its essential oils, which are in high demand and have a very important economicinterest, particularly for the pharmaceutical and cosmetic industries. This work had been done to study seedgermination and in vitro culture of Lavandula angustifolia. The seeds of L. angustifolia had undergonea vernalization followed by two treatments (physical and chemical), in order to increase the percentage ofgermination. Seeds were introduced into tubes containing the culture medium Murashige et Skoog (MS).Untreated seeds were germinated in the same medium supplemented with gibberellic acid at differentconcentrations. The kinetics, the speed, and the final germination rate were retained in order to evaluate theresponse of seeds. The micropropagation of L. angustifolia was performed on MS medium supplemented withdifferent concentrations of 6-Benzylaminopurine (Treatment I) and 6-Benzylaminopurine in combination withNaphthalene Acetic Acid (Treatment II). Multiplication rate, shoots number, nodes number, leaves number, andstem length were determined. Lavandula angustifolia seeds revealed very low germination percentages for allthe treatments used. However, those treated with sand (physical treatment) showed the highest percentage ofgermination (22%) followed by sulfuric acid treatment with a percentage of 14%. Results showed that the 11.11μM concentration of 6-Benzylaminopurine favored the maximum reactivity of the explants. The combinationbetween 6-Benzylaminopurine and Naphthalene Acetic Acid showed that MS supplemented with 8.88 μM6-Benzylaminopurine and 2.68 μM Naphthalene Acetic Acid was the most effective in the development ofvitroplants.
ABSTRACT
Abstract Asthma is an inflammatory disease of the lungs, and it causes oxidative stress. Lavandula dentata is an aromatic herb with anti-oxidative and anti-inflammatory activities. This study examined the activity of L. dentata extract on a guinea pig model of asthma. Adult males were divided into five groups: First group was control, second was asthma model induced by OVA, third was treated with L. dentata extract orally (300 mg/kg) for 21 days; the fourth was an asthma model with L. dentata extract (300 mg/kg) and fifth was treated with Tween 80 for 21 days. OVA treatment increased IgE, triglycerides, total cholesterol, glucose levels in serum, WBC count in blood and MDA in lungs. Also, OVA reduced SOD activity, GSH content in lungs, and GGT activity in serum (p<0.05). L. dentata extract treatment in asthma model reduced elevated IgE, triglycerides, total cholesterol, glucose levels in serum, and MDA in lungs (p<0.05), while it increased GSH content in lungs (p<0.05). These results suggest the possibility that L . dentata extract can exert suppressive effects on asthma, and may provide evidence that it is a useful agent for the treatment of allergic airway disease, it also limits oxidative stress induced by OVA. L. dentata extract appears to have hypolipidemic and hypoglycemic activities.
Resumo A asma é uma doença inflamatória dos pulmões e causa estresse oxidativo. Lavandula dentata é uma erva aromática com atividades anti-oxidantes e antiinflamatórias. Este estudo examinou a atividade do extrato de L. dentata em um modelo de asma de cobaia. Os machos adultos foram divididos em cinco grupos: o primeiro grupo foi controle, o segundo modelo foi o da asma induzido pela OVA, o terceiro foi tratado com extrato de L. dentata por via oral (300 mg / kg) por 21 dias; o quarto foi um modelo de asma com extrato de L. dentata (300 mg / kg) e o quinto foi tratado com Tween 80 por 21 dias. O tratamento com OVA aumentou a IgE, os triglicerídeos, o colesterol total, os níveis de glicose no soro, a contagem de leucócitos no sangue e o MDA nos pulmões. Além disso, o OVA reduziu a atividade da SOD, o conteúdo de GSH nos pulmões e a atividade da GGT no soro (p <0,05). O tratamento com extrato de L. dentata no modelo de asma reduziu a IgE elevada, triglicérides, colesterol total, níveis séricos de glicose e MDA nos pulmões (p <0,05), enquanto aumentou o conteúdo de GSH nos pulmões (p <0,05). Estes resultados sugerem a possibilidade do extrato de L. dentata poder exercer efeitos supressores sobre a asma, e pode fornecer evidências de que é um agente útil para o tratamento de doenças alérgicas das vias aéreas, além de limitar o estresse oxidativo induzido pela OVA. O extrato de L. dentata parece ter atividades hipolipemiantes e hipoglicêmicas.
Subject(s)
Animals , Male , Asthma , Lavandula , Bronchoalveolar Lavage Fluid , Plant Extracts , Ovalbumin , Disease Models, Animal , Guinea PigsABSTRACT
Objective: To investigate the lignans compounds constituents of Lavandula angustifolia. Methods: The chemical constituents were isolated and purified by TLC, silica gel, MCI-gel, and RP-HPLC, and their structures were identified by analysis of spectroscopic evidences and physicochemical properties. Results: A total of 11 constituents were isolated from L. angustifolia and elucidated as pinoresinol (1), syringaresinol (2), fraxiresinol-4'-O-β-D-glucopyranoside (3), syringaresinol-4'-O-β-D-glucopyranoside (4), 8-hydroxypinoresinol-4-O-β-D-glucopyranoside (5), rel-(2α,3β)-7-O-methylcedrusin (6), lariciresinol-4'-O-β-D-glucoside (7), (2S,3R)-2,3-dihydro-2-(4-hydroxy-3-methoxyphenyl)-3-hydroxymethyl-7-methoxybenzofuran-5-(trans) propen-1-ol-3-O-β-glucoside (8), (7S,8R)-dihydrodehydrodiconiferyl alcohol-9-β-D-glucopyranoside (9), (7R,8R)-7,8-dihydro-9'-hydroxyl-3'-methoxyl-8- hydroxymethyl-7-(4-hydroxy-3-methoxyphenyl)-1'-benzofuranpropanol-9'-O-β-D-glucopyranoside (10), and (E)-3-((2S,3S)-2-(4- hydroxy-3-methoxyphenyl)-3-hydroxymethyl-7-methoxy-2,3-dihydrobenzofuran-5-yl) allyl-2-hydroxyacetate (11). Conclusion: The 11 compounds are isolated from this plant for first time.
ABSTRACT
Candida sp. especially C. albicans is the main cause of candidiasis in women in reproductive ages. The prevalence of drug resistant C. albicans along with adverse effects of current treatments have encouraged the scientists to research on medicinal plant's essential oils. The aim of this study was to evaluate the potential effects of Ziziphora tenuior, Lavandula angustifolia, Cuminum cyminum essential oils against clinical isolates of C. albicans, which were isolated from women with vulvovaginal candidiasis. The anti-candidal effects of these commercial essential oils were screened against these clinical isolates (n=80) by disc diffusion and micro-broth dilution assays. The means of inhibition zone diameters, MIC and MFCs were reported for each essential oil. Also, the capability of fungal strain for biofilm formation in comparison with C. albicans ATCC 10231 were determined. The anti-biofilm effects of essential oils against 11 biofilm producing strains of C. albicans were determined. The results showed C. cyminum and Z. tenuior essential oils had the best anti-candidal effects, while the best biofilm killing effects was for L. angustifolia essential oil. C. cyminum and Z. tenuior essential oils can be formulated for more evaluations in preclinical and clinical studies.
Candida sp., especialmente C. albicans, es la principal causa de candidiasis en mujeres en edad reproductiva. La aparición de resistencia a los antifungicos de C. albicans, junto con el riesgo de efectos adversos de los tratamientos actuales, ha llevado a los científicos a buscar alternativas en los aceites esenciales derivados de plantas. Los objetivos del estudio fueron evaluar los efectos potenciales de los aceites esenciales de Ziziphora tenuior, Lavandula angustifolia, Cuminum cyminum, contra aislados clínicos de C. albicans, obtenidos de mujeres con candidiasis vulvovaginal. Los efectos anti-Candida de estos aceites esenciales comerciales fueron probados contra estos aislados clínicos (n=80) por difusión en disco y ensayos de microdilución. Se obtuvo el promedio de diámetro de inhibición, MIC y MFCs para cada aceite esencial. También se comparó la capacidad de formación de biopelículas de 11 cepas de C. albicans de cada aislado frente a la cepa de referencia C. albicans ATCC 10231 y la capacidad de cada aceite esencial para evitar la formación de biopelículas. Los resultados muestran que los aceites esenciales de C. cyminum y Z. tenuior tuvieron la mejor actividad anti-Candida, mientras que los mejores efectos para destruir biopelículas se obtuvieron con los aceites esenciales de L. angustifolia. Los aceites esenciales de C. cyminum y Z. tenuior ameritan tener mayores evaluaciones preclínicas y clínicas.
Subject(s)
Humans , Female , Adult , Candida albicans , Candidiasis, Vulvovaginal , Oils, Volatile , Biofilms , Cuminum , LavandulaABSTRACT
OBJECTIVES: The present systematic review was conducted to compare the effect of lavender on the quality of sleep, sexual desire, and vasomotor, psychological and physical symptoms among menopausal and elderly women. METHODS: There were five electronic databases which selected to search respective articles which included were Scopus, PubMed, Web of Science, Google Scholar, and the Cochrane Library without any language restriction since the study inception to March 10, 2018. The quality of studies was assessed in accordance with a jaded scale. RESULTS: According to three trials, the lavender as utilized in a capsule form or aromatherapy could significantly improve the measured Pittsburgh Sleep Quality Index score (P < 0.05) in the menopausal and elderly women. The aromatherapy with lavender improved sexual function (P < 0.001), depression (P < 0.001), anxiety (P < 0.001), and physical (P < 0.001) symptoms. Based on a trial, 66.7%, 70.0%, and 53.3% of subjects reported feelings of relaxation, happiness, and cleanness effects of having used lavender respectively. CONCLUSIONS: The results suggested the effectiveness of the use of lavender either in capsule form or aromatherapy on the improved quality of sleep, depression, anxiety, sexual desire, and psychological and physical symptoms. These results, however, should be interpreted with caution considering the limitations of the study.
Subject(s)
Aged , Female , Humans , Anxiety , Aromatherapy , Depression , Happiness , Hot Flashes , Lavandula , Libido , Menopause , RelaxationABSTRACT
PURPOSE: Although lavender is purported to possess anxiolytic and sedative properties and is often recommended for relieving anxiety, the efficacy of lavender has not been well established. Thus, this review aimed to evaluate the anxiolytic effects of lavender aromatherapy.METHODS: Ten data bases were searched for studies published between 2000 and 2018. Randomized controlled trials investigating the anxiolytic effects of lavender aromatherapy with any type of application for persons with or without clinical anxiety were included. The outcome variables included self-rated anxiety, vital signs, and salivary cortisol and chromogranin A (CgA) levels. In the meta-analysis, standardized mean difference and 95% confidence interval were calculated as effect measures by applying the random effect model and inverse variance method.RESULTS: Twenty-two trials met our inclusion criteria. Lavender aromatherapy was found to have favorable effects in relieving anxiety (Hedges' ĝ = −0.65; 95% CI, −0.84 to −0.46) and decreasing systolic blood pressure (ĝ = −0.22; 95% CI, −0.43 to −0.02), heart rate (ĝ = −0.53; 95% CI, −0.74 to −0.32), and salivary cortisol (ĝ = −1.29; 95% CI, −2.23 to −0.35) and CgA (ĝ = −2.29; 95% CI, −3.24 to −1.34) levels. However, the meta-analysis did not reveal any significant effects of lavender on diastolic blood pressure (effect size: −0.17; 95% CI, −0.37e0.04).CONCLUSION: Aromatherapy using lavender oil might have favorable effects on anxiety and its physiological manifestations. Future studies are recommended with an emphasis on methodological quality. In nursing practice, it is suggested that lavender aromatherapy be included in programs intended to manage anxiety in patients across diverse healthcare settings.
Subject(s)
Humans , Anti-Anxiety Agents , Anxiety , Aromatherapy , Blood Pressure , Chromogranin A , Delivery of Health Care , Heart Rate , Hydrocortisone , Lavandula , Methods , Nursing , Vital SignsABSTRACT
ABSTRACT: Lavender is an aromatic ornamental plant that is used widely in the perfume, pharmaceutical, cosmetical and food industries. That is why it is important to study ways to promote a production of lavender raw material. For this, four experiments were carried out in order to study the germination and initial in vitro development of lavender. In the first part the efficiency of the use of hydrogen peroxide in the pre-treatment of seeds inoculated in culture media with different concentrations of gibberellic acid was evaluated. Besides that, the influence of the use of Growlux lamps and white fluorescent lamps on plantlets cultivated in MS and LS media at salts concentrations of 50 and 100% was evaluated. Finally, the effect of the gibberellic acid, putrescine, spermine and spermidine in different concentrations was evaluated. Better results were estimated when hydrogen peroxide pretreatment was applied to the seeds with subsequent inoculation in a medium containing 2,5 mg L-1 of gibberellic acid. The use of Growlux lamps did not influence the characteristics observed when compared to the use of white fluorescent lamps, however it was concluded that the species develops more in a LS medium. Concerning the use of polyamines was found that germination, aerial part and number of leaves of the seedlings were generally favored at the concentration of 0,5 mg L-1 except when spermine was applied, which resulted in a lower number of leaves under this condition.
RESUMO: Lavanda é uma planta ornamental aromática que é amplamente utilizada nas indústrias de perfumaria, farmacêutica, cosmética e alimentícia e por isso é importante estudar maneiras de promover a produção dessa matéria-prima. Para tanto, foram realizados quatro experimentos com o objetivo de estudar a germinação e o desenvolvimento inicial in vitro da lavanda. No primeiro experimento testou-se a eficiência do uso de peróxido de hidrogênio no pré-tratamento de sementes inoculadas em meios de cultura com diferentes concentrações de ácido giberélico. Além disso, foi avaliado a influência do uso de lâmpadas Growlux e de lâmpadas fluorescentes brancas em plântulas cultivadas nos meios MS e LS em concentrações de sais de 50 e 100%. Por fim, também estudou-se o efeito dos reguladores ácido giberélico, putrescina, espermina e espermidina em diferentes concentrações. Melhores resultados foram encontrados quando o pré-tratamento com peróxido de hidrogênio foi aplicado às sementes com posterior inoculação em meio contendo 2,5 mg L-1 de ácido giberélico. O uso de lâmpadas Growlux não influenciou as características avaliadas quando comparado ao uso de lâmpadas fluorescentes brancas, entretanto observou-se que as espécies se desenvolvem melhor em meio LS. Com relação ao uso de poliaminas, verificou-se que a germinação, parte aérea e número de folhas das plântulas foram favorecidas na concentração de 0,5 mg L-1, exceto quando se aplicou espermina, que resultou em menor número de folhas nessa condição.
ABSTRACT
ABSTRACT: The essential oils of the different parts of Lavandula dentata L. (inflorescences and aerial part without inflorescences) collected in the city of Uberaba (minas Gerais State)were obtained by hydro distillation, and their chemical composition was determined by gas chromatography coupled to mass spectrometry and compared to the chemical composition of essential oil of Lavandula hybrida and Lavandula officinalis. It was observed that the essential oils of the studied species have varied chemical composition and are composed mainly of monoterpenes. The essential oils of L. hybrida and L. officinalis showed a higher concentration of linalool and linaline acetate, while L. dentata L. presented higher concentration of fenchone, eucalyptol and camphor. Results indicate that the essential oil composition of L. dentata L. grown in Uberaba is similar to those produced in Curitiba - PR, providing a promising perspective for the cultivation and extraction of essential oils of this species in Minas Gerais.
RESUMO: Os óleos essenciais das diferentes partes de Lavandula dentata L. (inflorescências e parte aérea sem inflorescência) coletados em Uberaba - MG foram obtidos por hidrodestilação, e sua composição química foi determinada por cromatografia gasosa acoplada a espectrometria de massas e comparada à composição química de óleo essencial de Lavandula hybrida e Lavandula officinalis. Observou-se que os óleos essenciais das espécies estudadas possuem composição química variada e são compostos, principalmente, por monoterpenos. Os óleos essenciais de L. hybrida e L. officinalis apresentaram maior concentração de linalol e acetato de linalina, enquanto L. dentata L. apresentou maior concentração de fenchona, eucaliptol e cânfora. Os resultados indicam que os óleos essenciais de L. dentata L. cultivada em Uberaba são semelhantes aos produzidos em Curitiba - PR, tornando-se uma perspectiva promissora para o cultivo e extração de óleos essenciais desta espécie em Minas Gerais.
ABSTRACT
OBJECTIVE:To establish the quality standard for Compound Lavandula angustifolia ointment. METHODS:TLC was used for the qualitative identification of ethanol extract from Scutellaria baicalensis and volatile oil of L. angustifolia. GC method was used for qualitative identification of dementholized peppermint oil. GC method was used to determine the content of menthol. The determination was performed on Agilent DB-WAX capillary column,with temperature programming. The injector temperature was 250℃,and the temperature of detector was 250℃.The injection volume was 1 μL and the split ratio was 5:1 by split sampling. RESULTS:TLC spots of ethanol extract of S. baicalensis and volatile oil of L. angustifolia were clear and well-repeated without interference from negative control. The chromatographic peaks in TLC of test samples of dementholized peppermint oil had same retention time as that of substance control.The linear range of menthol injection amount was 0.113 4-1.133 5μg (r=0.999 4). RSDs of precision,intra-day precision,stability and reproducibility tests were not higher than 2.0%. The recoveries were 95.40%-99.82%(RSD=1.61%,n=6). CONCLUSIONS:Established quality standard can be used for the quality control of Compound L.angustifolia ointment.
ABSTRACT
Abstract Asthma is an inflammatory disease of the lungs, and it causes oxidative stress. Lavandula dentata is an aromatic herb with anti-oxidative and anti-inflammatory activities. This study examined the activity of L. dentata extract on a guinea pig model of asthma. Adult males were divided into five groups: First group was control, second was asthma model induced by OVA, third was treated with L. dentata extract orally (300 mg/kg) for 21 days; the fourth was an asthma model with L. dentata extract (300 mg/kg) and fifth was treated with Tween 80 for 21 days. OVA treatment increased IgE, triglycerides, total cholesterol, glucose levels in serum, WBC count in blood and MDA in lungs. Also, OVA reduced SOD activity, GSH content in lungs, and GGT activity in serum (p 0.05). L. dentata extract treatment in asthma model reduced elevated IgE, triglycerides, total cholesterol, glucose levels in serum, and MDA in lungs (p 0.05), while it increased GSH content in lungs (p 0.05). These results suggest the possibility that L . dentata extract can exert suppressive effects on asthma, and may provide evidence that it is a useful agent for the treatment of allergic airway disease, it also limits oxidative stress induced by OVA. L. dentata extract appears to have hypolipidemic and hypoglycemic activities.
Resumo A asma é uma doença inflamatória dos pulmões e causa estresse oxidativo. Lavandula dentata é uma erva aromática com atividades anti-oxidantes e antiinflamatórias. Este estudo examinou a atividade do extrato de L. dentata em um modelo de asma de cobaia. Os machos adultos foram divididos em cinco grupos: o primeiro grupo foi controle, o segundo modelo foi o da asma induzido pela OVA, o terceiro foi tratado com extrato de L. dentata por via oral (300 mg / kg) por 21 dias; o quarto foi um modelo de asma com extrato de L. dentata (300 mg / kg) e o quinto foi tratado com Tween 80 por 21 dias. O tratamento com OVA aumentou a IgE, os triglicerídeos, o colesterol total, os níveis de glicose no soro, a contagem de leucócitos no sangue e o MDA nos pulmões. Além disso, o OVA reduziu a atividade da SOD, o conteúdo de GSH nos pulmões e a atividade da GGT no soro (p 0,05). O tratamento com extrato de L. dentata no modelo de asma reduziu a IgE elevada, triglicérides, colesterol total, níveis séricos de glicose e MDA nos pulmões (p 0,05), enquanto aumentou o conteúdo de GSH nos pulmões (p 0,05). Estes resultados sugerem a possibilidade do extrato de L. dentata poder exercer efeitos supressores sobre a asma, e pode fornecer evidências de que é um agente útil para o tratamento de doenças alérgicas das vias aéreas, além de limitar o estresse oxidativo induzido pela OVA. O extrato de L. dentata parece ter atividades hipolipemiantes e hipoglicêmicas.
ABSTRACT
Lavandula angustifolia (Family Labiates) is a medicinal herb found in Mediterranean area. It is a well known herb in ayurvedic system of medicines and has traditionally been used to treat disorder of liver, fever and several conditions including infertility, infection and anxiety. There are few reports on tissue culture of Lavandula angustifolia that too mainly on micropropagation. Present study explored an in vitro micropropagation of Lavandula angustifolia. In vitro callus formation was established by using nodal segments on Murashique and Skoog, (1962) medium (MS) supplemented with IAA at 0.1mg/l, l BAP at 0.002mg/l and 2-4D at 0.2mg/l, significantly recorded complete callus formation after 6 weeks of incubation at 25±1ºC. The callus was allowed for organogenesis and then shoot multiplication was carried out at 4 concentrations of BAP (0.5, 1, 2 and 1mg/L) and IAA (0.5, 0.5, 0.5 and 1mg/L) on MS medium. The shoot regeneration medium for shoot multiplication and proliferation with higher number of shoots was recorded at 0.5mg/l of IAA and 2.0mg/l BAP. However, the growth was very steady and originating from the base. MS medium without any growth regulators tabulated the tallest shoot length of 35 mm, but the shoots were clustered not properly differentiated.
ABSTRACT
OBJECTIVE@#To evaluate toxic activities of essential oils obtained from Rosmarinus officinalis and Lavandula stoechas against the fourth larval instars of Orgyia trigotephras.@*METHODS@#A total of 1 200 larvae were divided into three groups I II III. Group I was to investigate the effect of extracted essential oils from these aromatic plants as gastric disturbance. Bacillus thuringiensis and ethanol were used as control group. Group II was used as contact action and Group III was used as fumigant action. Decis and ethanol were used as control group. During the three experiments, the effect of essential oils on larvae was assessed.@*RESULTS@#The chemical composition of essential oils from two medicinal plants was determined and, their insecticidal effects on the fourth larval state of Orgyia trigotephras were assessed. The two simples presented an insecticidal activity, nevertheless Rosmarinus officinalis essential oil was less efficient compared to Lavandula stoechas one are discussed.@*CONCLUSIONS@#The relationship between the chemical composition and the biological activities is confirmed by the present findings. Therefore the potential uses of these essential oils as bioinsecticides can be considered as an alternative to the use of synthetic products.
ABSTRACT
Objective:To evaluate toxic activities of essential oils obtained fromRosmarinus officinalis andLavandula stoechas against the fourth larval instars of Orgyia trigotephras.Methods:A total of1200 larvae were divided into three groups 栺,栻,栿.Group 栺 was to investigate the effect of extracted essential oils from these aromatic plants as gastric disturbance.Bacillus thuringiensis and ethanol were used as control group.Group 栻 was used as contact action and Group 栿 was used as fumigant action.Decis and ethanol were used as control group.During the three experiments, the effect of essential oils on larvae was assessed.Results:The chemical composition of essential oils from two medicinal plants was determined and, their insecticidal effects on the fourth larval state ofOrgyia trigotephras were assessed.The two simples presented an insecticidal activity, neverthelessRosmarinus officinalis essential oil was less efficient compared toLavandula stoechasone are discussed.Conclusions:The relationship between the chemical composition and the biological activities is confirmed by the present findings.Therefore the potential uses of these essential oils as bioinsecticides can be considered as an alternative to the use of synthetic products.
ABSTRACT
Objective To establish the fingerprints of 20 batches of Lavandula Angustifolia by HPLC. Methods The determination was performed on a Phenomenex ODS-A column (250 mm× 4.6 mm, 5 μm). The mobile phase was in gradient elute mode with a mixture consisting of acetonitrile and 0.036 mol/L phosphate acid solution. The flow rate was 1.0 mL/min. The temperature was 30 °C. The determine wavelength was 350 nm. The fingerprints of 20 batches of Lavandula Angustifolia were compared and classified by similarity evaluation, cluster analysis, and principal composition analysis. Results Totally 10 chromatographic peaks were extracted as the common peaks of Lavandula Angustifolia, and 2 peaks were identified. The similarity degrees of the 20 batches of Lavandula Angustifolia were above 0.9. All the batches of Lavandula Angustifolia were classified into 3 categories. Conclusion The method is simple and reproducible, and can be used for the standardization and quality control of Lavandula Angustifolia.
ABSTRACT
Objective: To evaluate toxic activities of essential oils obtained from Rosmarinus officinalis and Lavandula stoechas against the fourth larval instars of Orgyia trigotephras. Methods: A total of 1 200 larvae were divided into three groups I II III. Group I was to investigate the effect of extracted essential oils from these aromatic plants as gastric disturbance. Bacillus thuringiensis and ethanol were used as control group. Group II was used as contact action and Group III was used as fumigant action. Decis and ethanol were used as control group. During the three experiments, the effect of essential oils on larvae was assessed. Results: The chemical composition of essential oils from two medicinal plants was determined and, their insecticidal effects on the fourth larval state of Orgyia trigotephras were assessed. The two simples presented an insecticidal activity, nevertheless Rosmarinus officinalis essential oil was less efficient compared to Lavandula stoechas one are discussed. Conclusions: The relationship between the chemical composition and the biological activities is confirmed by the present findings. Therefore the potential uses of these essential oils as bioinsecticides can be considered as an alternative to the use of synthetic products.